Sentence examples for backbone was religated to from inspiring English sources

The resulting intermediate plasmid was then digested with XbaI to liberate the yeast helper fragment, and the backbone was religated to yield pCRISPomyces-1.

To create the plasmids used in this study, the promoter-TNR URA3 region of pBL252 was removed by BamHI and XhoI digestion, and the backbone was religated with a BamHI- XhoI promoter-TNR CAN1 fragment.

In order to remove one of the two cer regions, the plasmids pJOE5058.1 and pWA19 were cut with MluI, a 264 bp-fragment was excised, the vectors were religated to obtain the plasmids pWA21 and pWA22, respectively.

The results clearly indicated that when ChIP material was religated in presence of stuffer DNA (improved MDA), it allowed to recover the original pattern, while standard MDA and MDA without stuffer DNA failed to do so.

pVT1 digested by NotI was religated between the two NotI sites of pBeloBac11 [33], giving pBac17.

The plasmid was religated using DNA T4 ligase.

The religation step is precluded, because the dinucleotide, generated during cleavage, is too short to be religated, leaving the enzyme covalently attached to the 3′ end of the oligonucleotide.

Molars were religated every 2-3 days to ensure retention.

ADR inhibits the progression of the enzyme topoisomerase II after it has broken the DNA chain to allow replication; it prevents the DNA double helix from being religated [86], [87].

The bacterial RuvC enzyme is able to symmetrically cleave HJs in vitro and generates products that can be religated without further processing.

Methylated and mock-methylated plasmids were religated into SacI/HindIII-restricted pGL4.10-Basic vector [49].