Sentence examples for backbone was performed using from inspiring English sources
Theoretical O-base oxidation in protein backbone was performed, using an alanine peptide as a model, and focused on the peptide backbone.
Using the assumption that the hGBE1-apo crystal structure represents an active enzyme conformation, the design of an hGBE1 p.Y329S-stabilizing peptide was performed using a rigid backbone modelling of the mutation, in order to retain maximum similarity to the active enzyme.
In addition, besides studying the complete secondary metabolite network a focused study on terpenoids backbone biosynthesis (TBB) pathway using mevalonate and non-mevalonate pathway was performed using KEGG (map00900).
PCR products were subcloned into pCR 2.1-TOPO vector using the TOPO TA Cloning Kit (Invitrogen) and sequencing was performed using the M13R primer that localizes to the vector backbone.
To add 2OG and Fe(II) to the predicted structure and the predicted active site of Jmjd6, an iterative backbone annealing of the Hif1an structure (1h2k) on top of the Jmjd6 model was performed using the SwissPDB Viewer [ 102].
The absent backbone atoms and side chains of each intermediate structure were reconstructed using Maxsprout [ 28], and energy minimization was performed using Swiss-PDB viewer [ 29].
The docking method was performed using a two-step process of rigid-body Monte Carlo searching and parallel optimization of the backbone displacement and side-chain conformations.
Additional molecular modeling was performed using a modified version of a more recently released template of a tandem pore potassium channel with a different amino acid backbone connectivity.
Manual backbone and side chain refitting were performed using Coot [46].
While most of the known biology is represented in the visible backbone of the networks, network computations can be performed using a downstream (hidden) layer of RNA abundance of genes regulated by the nodes in the backbone.
