Sentence examples for backbone was excised from from inspiring English sources
The ITR-containing pAAV vector backbone was excised from the pAAV-MCS plasmid (Stratagene) by cleavage with NotI and subsequent dephosphorylation by calf intestinal alkaline phosphatase (NEB).
The pGEM backbone was excised from pHTK [34] by digesting with Nar I- Not I, and pGEM and the components described above were assembled into the pSTCII-GFP plasmid through several cloning and subcloning steps.
pBI-EYFPBax-TetO-ECFPBax was excised from the plasmid backbone by PshAI/AseI digestion.
The full-length Dazl promoter was excised from the pGL3 vector backbone with SmaI/HindIII and subcloned into the pBluescriptII-KS intermediate cloning vector, introducing BamHI and XhoI sites flanking the promoter sequence.
To generate the FSMAD1 transgene construct, the Flag-SMAD1 cDNA was excised from pCMV5-FlagSMAD1 [33] and cloned 3' to a loxP-flanked neomycin resistance cassette with 3 polyadenylation-sites (the backbone plasmid is a kind gift from Drs. A. Simeone and F. Tuorto, Institute of Genetics and Biophysics, Naples).
The MEPE cDNA was excised from the pEN.T-MEPE sub-cloning vector using BamHI and XbaI and ligated into pLZ2-Ub backbone to create a Ubiquitin driven MEPE expression construct, pLZ2-Ub.MEPE.
Fourteen transformants stably carried the KJhyPBase expression cassette, and TR_cKJUMPS was excised from seven of these.
