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The main chiral backbone used for these studies was 1,2- trans-cyclohexanediamine (5) (Scheme 1).[ 14 ] After mono-Boc-protection,[ 15 ] quaternarization could be achieved either by treating 6 with an excess of methyliodide (to obtain trimethylammonium salt-based intermediates 7) or by carrying out a reductive amination with different arylcarbaldehydes first, followed by methylation.
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We reason the different response to PRL of reporters is due to the limited proximal promoter regions utilized, the different backbones used for reporter construct, and cells lines used for reporter assay.
The carbon skeleton backbones used for amino acid biosynthesis in plants are derived from organic acids produced in glycolysis, the oxidative pentose phosphate pathway and the TCA and Calvin cycles.
The model was adjusted for the backbone used, age, sex, and years since HCV infection at cohort entry.
Unlike the present study, these studies were not restricted to coinfected persons, allowed for switches in the class of anchor agent, and failed to account for the backbone used.
The same vector backbone was used for SK expression.
Linearized pSB1C3 backbone was used for all cloning, and was obtained as a part of iGEM 2013 DNA Distribution Kit.
The generated targeting vector (TK-KanaGalK-TK) (Fig. 2a) was excised out from the plasmid backbone and used for heat-inducible homologous recombination in SW102 E. Coli containing the BAC BoHV-4 genome.
All three pJ20x plasmid backbones were used for Biobrick2 standard assembly to create all desired DNA fragments for integration into the C. acetobutylicum genome [list of BB2 plasmids in Additional file 2].
The 454 reads were assembled using Seqman Ngen to generate a backbone; subsequently, the Illumina reads were mapped onto this backbone using Seqman Ngen to correct for technology-inherent read errors.
Data for the conserved sequence segments in the output backbone files were used for constructing Oxford grids.
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