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The MEPE cDNA was excised from the pEN.T-MEPE sub-cloning vector using BamHI and XbaI and ligated into pLZ2-Ub backbone to create a Ubiquitin driven MEPE expression construct, pLZ2-Ub.MEPE.
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DNA glycosylases remove damaged bases from the DNA backbone to create an AP site.
Lastly, we have expressed dominant-negative Tcf4 (dnTcf4) or constitutively active beta-catenin (β-catenin4A) from the hEF1α promoter in a SV40-PuroR or SV40-mCherry backbone to create vectors that inhibit or activate the Wnt signaling pathway.
Reading about the lore of certain events and how they happened can give you a good backbone to creating your character.
Boards also matter because systematically, strong corporate governance forms the backbone to creating diverse, sustainable and scalable businesses that make an impact in our world.
In the new study, Kawaoka's team randomly mutated the genes in the backbone of the virus to create a large library of variants and then tested them with a wide variety of different surface proteins.
Move the knife along the backbone of the chicken to create a slit.
Copolymer based on acrylic acid derivatives containing mesogenic groups in the side chains and thiol group at the end of the backbones has been synthesized to create a liquid crystal cholesteric composite with the gold nanoparticles (NPs).
Facebook's founder, Mark Zuckerberg, said last month that the goal was to "create a backbone for many things, including access to information, access to information about health care, education, jobs and just so many good things".
It is supposed to create a backbone for developing the middle part of peninsula by facilitating the transport of mineral resources and accessibility to such region and encourage populating the remote desert city of Nekhel and the coastal cities of Taba and El Shatt.
The idea is to create a backbone in the suburban Bloomington-Normal area, and then branch out to surrounding rural communities.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com