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In a previous comprehensive study on Peronospora phylogeny [41], backbone resolution of the phylogenetic trees was relatively low.
Two positive integrants as determined by colony pcr and FIGE were further processed for backbone resolution.
Diagnostic restriction digests and FIGE was further carried to verify the genomic integrity of the clones and confirm pLD53 backbone resolution.
The phylogenetic analysis described above of the Sec24 tree (supplementary fig. S3, Supplementary Material online) failed to show backbone resolution between paralogs.
This provided moderate support for TSET as a clade, but strong resolution excluding it from the APs and COPI, as well as backbone resolution between the heterotetramer clades.
Considering only supported clades (PB > 70%, LB > 70% and PP > 95%), the backbone resolution of the phylogenetic tree generated from the combined dataset was higher than the tree inferred from ITS.
Similar(52)
HDX examines the effects of dimerization based on changes in peptide backbone solvent accessibility, with spatial resolution limited by digestion coverage and peptide size, whereas site-directed mutagenesis with AUC measures the energetic contributions of individual residues to dimerization.
Determination, resoluteness, resolution, resolve, firmness (of purpose), steadfastness, backbone, drive, push, thrust, enthusiasm, ambition, initiative, enterprise, motivation, single-mindedness, commitment, conviction, dedication; get-up-and-go.
Using multidimensional NMR, we solved the structure of this intermediate mimic to an atomic resolution (backbone rmsd, 0.51 Å).
Then a second PASD calculation was done, in which the likelihood estimates were determined using the medium resolution backbone fold obtained previously for KTX in the solid phase (PDB CODE: 1XSW).[11] In all cases, the structures obtained from the two different PASD calculations were indistinguishable.
Here we have not used the ensemble of structures present at the end of pass 1 and 2 for calculation of likelihood estimates, but either the high-resolution structure of KTX obtained under different conditions or a medium-resolution backbone fold.
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