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To further dissect the backbone requirements for the tracrRNA and determine if any DNA residues could be tolerated, regions of the tracrHyb were reverted to RNA and tested for restoration of activity.
This has the potential to leave the operators vulnerable in their ability to service their existing wholesale and enterprise customers, whose backbone requirements typically consist of three diverse routes providing 'meshed' coverage into and out of a country from a network planning perspective.
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These are subsumed in the backbone of requirements.
The key geometrical elements of the nullclines can be described by a set of exclusive parametric combinations defined in a string, which we call the backbone of requirements.
The cases in (a-c) display the same geometrical shape and can be described by a single backbone of requirements shown in (d).
For the system we present here, only those mutations cause functional change, which induce a qualitative alteration in the shape of the nullclines, represented in the Backbone of requirements for the FFL response (BR) [ 26].
However, by analyzing the geometrical features of the nullclines it is possible to determine the so-called Backbone of Requirements for the FFL response (BR), i.e. a set of qualitative relationships between different geometrical features of the nullclines and the location of the initial and the final point that univocally determines the dynamics [ 26].
With the widespread use of broadband access technologies and the development of high-speed Internet backbones, the requirement for high-performance metropolitan area networks (MANs) is increasing.
Also with the droop control as backbone, the communication requirement (such as speed and bandwidth) can be reduced and failure of the communication links will not cause a system collapse.
Both conduct transesterification reactions of the DNA phosphodiester backbone without a requirement for high-energy cofactors such as ATP.
Specialty modules, including ICU, can be added to this backbone depending on mission requirements.
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