Sentence examples for backbone portion from inspiring English sources

Tol2 sequence elements were then recombineered into the plasmid backbone portion of the BAC, together with a neomycin resistance cassette.

Probes can be designed against either strand of genomic DNA, and the backbone portion can also be varied.

For all of the structures, the backbone portion of the amino acid is held in place by a hydrogen bonding network that is conserved between the two proteins.

One of the most conserved residues among the second-generation enzymes is Gln155, which is involved in interactions with the backbone portion of the amino acid substrate.

The His-tagged beta appendage domain expression construct is pGH491: Sequence encoding the C-terminus of mouse AP2 beta 1 (amino acids 592 951) was amplified using oGH1161-2 and recombined with the His-tag, T7 promoter and vector backbone portion of the His-tagged mu domain construct (pGH441 amplified with pGH571 and oGH339) using Gibson assembly.

Using the pRED-ET/RPSL-Neo counter selection system (Gene Bridges) the TetO array was seamlessly recombineered into BAC RP11 419E6, which contains approximately 170 kb of DNA corresponding to a gene desert on human chromosome 7. Tol2 sequence elements (Shakes et al., 2011 ) were then recombineered into the plasmid backbone portion of the BAC, together with a mammalian neomycin resistance cassette.

Organic anions (organic acids) and organic cations (organic bases) are collective terms for substances that possess a carbon backbone and a portion with net negative or positive charge in their basic structure.

The methods have often been successful in rapidly and sustainably achieving better outcomes and now form the backbone of major portions of professional and organisational credentialling.

These less ordered dimer conformations are characterized by a large number of mutual contacts and a significant portion of backbone hydrogen bonds.

It also confirms a proposal that a Med7C-Med21-Med4-Med9 tetramer structurala structural backbone within the central portion of the Middle module (Lariviere et al., 2013).

These two residues are on opposite sides of a hairpin loop in the β-sheet forming the backbone of the NBD2 portion of the major catalytic MgATPase site 2 in SUR1.