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In binary and ternary complexes of KIX, the three-helix scaffold of the protein is well-ordered in solution, with backbone order parameters of ca. 0.9 for helices α1−α3.
This guarantees that the backbone order of matched nucleotides as well as base pairs of the given RNAs are preserved.
TALOS-N also derives side-chain χ1 angle information and reports an estimated backbone order parameter S derived from the chemical shifts.
Likewise, the 1489 cm 1 component of the symmetric thiophene C=C stretch derives intensity from ordered PBTTT chains in bimolecular crystals, where side group and backbone order is improved.
These chemical shifts were used to obtain backbone torsional angles using TALOS (Torsion Angle Likelihood Obtained from Shift and sequence similarity), specifically the version TALOS-N 65) TALOS-N 65o derives side-chain χ1 angle information), and report an estimated backbone order parameter S derived from the chemical shifts.
If the backbone were even somewhat ordered, one would expect substantial chemical shift changes upon interaction, as well as broadening due to the increased spectral density at zero frequency (J 0)), even if the backbone order parameter, S^2, were as low as, say, 0.5.
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Two kinds of events affect genome organization: on one hand translocations and recombinations change the relative position of genes shared by two genomes (i.e. the backbone gene order); on the other, insertions and deletions leave the backbone gene order unchanged but they alter the gene neighborhoods by breaking the syntenic regions.
Besides, the RS-BS can exchange context info on the backbone in order to accelerate the handover process.
With the recent rapid improvement of wireless communication technology, wireless network gains increasing interest as a train backbone in order to provide the passenger service as well as the original real-time control duties.
Solid-state 13C NMR experiments were performed on a series of linear poly ethylene-co-acrylic acid) (E-AA) copoly ethylene-co-acrylicylic acid groupoly ethylene-co-acrylicndomly acidg the polyethylene bacopolymersorder to determine the impact that molar percent acid content and acid group spacing have on the structure and dynamics of these materials.
All AONs consisted of 2'- O-methyl RNA and had a phosphorothioate backbone in order to resist endonucleases and RNaseH degradation.
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