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The pGL3-Basic or Empty vector was used as a control to check if any of the co-transfected anti-miRNAs had background interaction with the backbone of the vector.
First, a minimal backbone of the vector pCMX2.5-hIgG1Fc-XP was generated.
The backbone of the vector (3 kb) was amplified from the plasmid pMF272 using primers pSC-tul1D-F and pSC-tul1D-R.
The backbone of the vector (3 kb) was amplified from the plasmid pMF272 using primers pSC-sah2D-F and pSC-sah2D-R.
The backbone of the vector (3 kb) was amplified from the plasmid pMF272 [ 50] using primers pSC-pyr4D-F and pSC-pyr4D-R.
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The backbone of the vectors is derived from the pUC vector series, which insures a high DNA yield during plasmid amplification.
The backbone of this vector is derived from the pCambia series of binary vectors for Agrobacterium-mediated plant transformation.
Furthermore, the backbone of this vector contains a thymidine kinase (TK) expression cassette, to allow negative selection against unwanted integration events.
In order to compare the novel pEPito-backbone with the backbone of the original vector, pEPI-1, a series of 13 novel vectors was constructed.
In addition, numerous commonly used restriction enzyme sites exist on the backbone of the binary vector, limiting the construction strategy with regard to the use of restriction sites.
Among these mutants, most were single nucleotide or short fragment-indels, while long fragment-inserts derived from the backbone of the gRNA vector were also observed.
More suggestions(15)
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backbone of the family
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backbone of the operation
backbone of the industry
backbone of the vote
backbone of the system
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backbone of the city
backbone of the country
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backbone of the novel
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