Sentence examples for backbone of the expression from inspiring English sources

To construct such an activity-directed selection system, the prk gene from Synechococcuselongatus PCC7942 together with a tryptophan-regulated promoter trpR-Ptrp were inserted into the backbone of the expression plasmid 2 to generate the selection plasmid.

The backbone of the expression plasmid was derived from pASK-IBA2 (IBA GmbH, Germany), which contains a tetracycline based promoter and an OmpA leader sequence for export into the periplasm.

Here we demonstrate that mtr-miR159b is effectively processed from its precursor molecule and thus represents a highly suitable backbone for the expression of amiRs in M. truncatula.

The backbone of the indicated expression vectors was typically derived from pQE80 (Qiagen, Hilden, Germany).

The radiation-inducible, control plasmid, pRA-ZsG was created from the pUC backbone of the prokaryotic expression plasmid pZsGreen (Clontech, Mountain View, CA, USA) by subcloning the RecA promoter into the SapI and NcoI sites to substitute the lac promoter.

The background fluorescence signal provided by the split YFP system was evaluated by cloning the split YFP N-terminal fragment upstream to an HA tag (N-YFP HA) aN-YFP HA-terminandfragmentheC-terminalependently in the backbone ofragmentFP expression vector.

The cassette was designed to allow easy exchange of the sequences to be expressed and easy removal of the vector backbone from the expression cassette.

Tendons cells link muscles to the backbone of the organism, and Gcm expression in these cells is required for proper muscle attachment (Soustelle et al. 2004).

A) Expression vector pET21a used as backbone to insert the expression cassette; B) Expression cassette comprised of a signal sequence (Ss), a HIS-tag, the thioredoxin gene end the ClavMO peptide.

The viral backbone of our lentiviral expression constructs is pLL3.7.

Interestingly, genes with higher expression in the carcinoma tumors, but with a generally lower level of expression, are the backbone of the favored actin-cytoskeleton activity.