We used a steered molecular dynamics simulation to explore the compliance properties of the backbone of surface-exposed residues in several model proteins: interleukin-2, mouse double minute protein 2 and proliferating cell nuclear antigen.
The unfused and non-self-cleaving Mxe GyrA intein-fused pCT4Re vectors were used as a backbone for surface display of the scFvs.
From the mid-1960s tHawkawk provided the backbone of U.S. surface-based air defenses in Europe and South Korea and was exported to many allies.
In contrast to the stalks, backbones of the surface exposed CDR3β loops showed greater variability.
All of the anchoring functional groups (-COOH) were completely used for connecting polymer backbone to the surface of TiO2, while free carboxylates not participating in bonding were observed with direct polymer adsorption on TiO2.
NMR studies have indicated the rate of conformational fluctuations of the protein backbone, in several surface loop regions, coincidence with the substrate turnover step [14], [26].
Comparing the resulting transient pockets revealed fundamental differences in the number of pockets, their volume, their polarity and reflected the intrinsic influence of the backbone movements on the formation of surface pockets.
While MyMiniLife eventually agreed to a deal that formed the backbone of FarmVille, discontent soon surfaced.
Pseudo-amphoteric BioCube media are readily immersible, but after complete immersion, they gradually become hydrophobic, as the polyethylene oxide modified PDMS is designed to alienate from polyurethane backbone of BioCube to provide hydrophobic surfaces exhibiting more affinity for bacterial attachment.
These materials have a neutral dextran backbone but different substitution patterns and thus enable a variation of surface charge (DEX neutral, CMD negative, DEAE positive).
The phosphate backbone of DNA provides a negatively charged surface for the binding of basic side chains (Lys, Arg) of proteins.
