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We obtained a Calpha backbone model for each subunit from an initial homology model refined against the cryo-EM density.
Greater accuracy of the flexible backbone model results from both increases and decreases in side-chain flexibility relative to the fixed-backbone model.
Results are obtained for a segmented, flexible backbone model of a typical naval frigate.
As such, this study focuses on using a multivariate data analysis techniques, to develop a RMSW load-displacement (backbone) model.
We show that such a gradient elasticity backbone model can be combined with any standard nonlinear constitutive driver to render a regularised model for softening inelasticity.
A flexible backbone model was designed with five cut-outs on the top side of the aluminum beam to realize approximately torsional stiffness as well as vertical and horizontal bending stiffness.
Similar(43)
We designed 135,000 artificial sequences for a repertoire of backbone models representing all theoretically possible axial rotation states.
Aided by the 3.96 Å density map of CI, we were able to accurately assign all the 14 core subunits together with 20 supernumerary subunits, and build 17 additional backbone models into the unoccupied density.
Rosetta assembles these fragments into full-sized protein backbone models using various energy terms and filters.
This step is then followed by all-atom refinement of the peptide-protein conformation with fine backbone modeling and side-chain placement, based on the Rosetta FlexPepDock refinement protocol [26].
Next we show the potential of MOTIF-EM to build Cα backbone models for cryoEM maps in special cases.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com