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We then independently transplanted the 2F5 epitope onto three of the same parent scaffolds using the newly developed backbone grafting procedure.
Crystal structures of side-chain and backbone grafting designs showed close agreement with both the computational models and the desired epitope structure.
In two cases, backbone grafting scaffolds bound antibody 2F5 with 30- and 9-fold higher affinity than corresponding side-chain grafting designs.
Backbone grafting of linear motifs is a general method to transplant functional motifs when backbone remodeling of the target scaffold is necessary.
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The chelating o-phen ligands project perpendicularly above and below the undulating layer. 1 represents the first example of 2D inorganic vanadium arsenate backbone grafted with the directly coordinated organic ligands.
Representative peaks at 1730, 1374 and 1379 cm−1 which corresponds to free carbonyl stretching vibration (C=O) and to CH3 bending deformation can confirm the presence of a polymer backbone grafted to the NPs.
Also, as SiO2 sample was used as background prior the SiO2 POOH analysis, any of Si O Si at 1098 cm−1 and Si OH at 793 cm−1 linkages could not appear into the SiO2 POOH spectrum and therefore, it is expected that only linkages corresponding to the –POOH backbone grafted to the SiO2 surface could be observed [24].
Psy-g-PMAIII (Fig. 3b) in addition to the above zones of weight loss, have two extra zones of weight loss 7.69 % between 25 205 °C and 48.29 % up to 750 °C, due to the breakdown of backbone grafted psyllium and PMA chains.
The brush was synthesized by a "grafting from" method and composed of poly 2-hydroxyethylmethacrylate) (poly 2-hydroxyethylmethacrylateoly(t-butyl acrylate)(P-HEMA chains.
We report a novel method to prepare graft copolymers of PS-g-PEGM with controlled backbone length, grafting sites and spacing length.
The latter consisted of a PAA backbone covalently grafted with C12 moieties at 1% mol (referred to as PAA-1C12).
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