Sentence examples for backbone fusion from inspiring English sources

The variable domains of mAb 41D3 were sequenced and cloned into the pVITRO1-neo-mcs vector (Invivogen), in frame with a mouse IgG1 backbone (Fusion Antibodies Ltd).

BsaI reopens the level 2i-1 bandbone and provides defined fusion sites which are compatible with the level 1 modules released by BpiI.

Backbone plasmids for TDmKate2-fusion were constructed based on mKate2-N1 and mKate2-C1 (the latter one was constructed by amplifying mKate2 from mKate2-N1 mKate2 FP1 and mKate2 RP2, and inserting it into peGFP via AgeI and XhoI sites).

We also note that recent studies indicate that IgA (Bakema et al, 2011; Bakema & van Egmond, 2011b; Lohse et al, 2011), IgE (Karagiannis et al, 2011) and IgM (Ammann et al, 2012; Kaveri et al, 2012) may also serve as alternatives to the classic IgG backbone for therapeutic Fc-fusions.

All of the IFP backbone fission sites discovered using laboratory evolution were distal from the BV-binding site, suggesting that IFP fission and fusion near this chromophore binding site is disruptive to fragment complementation.

Starting from zero expression we could boost the yields of active protein by optimization of codons, vector backbone, and a novel chaperone fusion system.

The plasmid backbones contain loxP sites, which allows fusion of pDyn1 and pDyn2 using Cre recombinase to create pDyn3.

All PCR products were ligated into pGEM T-easy vectors and digested with their corresponding restriction endonucleases (Fig. 4b), before fusion with the hrGFP vector backbone.

The resulting recombination inserted shuttle vector sequences containing the expression cassette and pIX-MT fusion gene into the pAdEasy1 adenovirus backbone replacing the E1A and E1B sequences.

ORCTL3-ECFP was generated as a fusion construct with a pcDNA3 vector backbone.

The construction of pOPIN-fusion vector backbones has been described elsewhere [ 51– 51].