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Other parameters, including the Backbone, frequency response function (FRF) and Q-value curves, are calculated from the free damped oscillation signals.
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The marker-free transformation was compared to kanamycin resistance assisted transformation in terms of T-DNA and vector backbone integration frequency.
We did not find significant differences in vector backbone integrations frequency when the different varieties or both of the marker-assisted transformation constructs were compared (Table 7).
Only when vector backbone integration frequency was compared between the marker-free (24%; Table 2) and marker-assisted transformation experiments (57%; Table 5) we found that marker-free transformation was associated with less vector backbone integration.
Backbone resonance frequencies, relaxation rates and solvent exposure measurements concur in showing this domain to adopt a helical conformation, with two helical segments spanning residues 717 726 and 732 746 connected by an unstructured linker containing the charged residues D728 and R730 involved in E1 binding.
Finally, we compared vector backbone integration frequencies among the different marker-free and marker-assisted transformation experiments.
Transformation and vector backbone integrations frequencies are binary data and, therefore, the Pearson Chi-square test was chosen to compare the independent samplings of transformation events in the different transformation experiments.
Although the S1 S2 transition is accompanied by a number of changes in carboxylate and backbone vibration frequencies as detected by infrared spectroscopy, the associated structural changes are most likely too small to be detected by the resolution achieved in the present study of ∼5 Å.
Further numerical simulations on the analytical model identify the nonlinear resonant frequency backbone curves for a range of system parameters.
Both the accuracy of the backbone and the frequency of NOS3 as the node with the largest BC value decrease rapidly when the number of omitted genes is 3 (Table 8 and Figure 6).
One peak at lower frequency reflected backbone relaxation and the other at higher frequency reflected branch relaxation.
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