Sentence examples for backbone for expression from inspiring English sources

pBabeHygro [28] was used a vector backbone for expression of Cre recombinase as described [3].

However, existing viral expression systems generally require different vector backbones for expression of cDNA, small hairpin RNA (shRNA) or microRNA (miRNA) and provide limited drug selection markers.

p1.2-Mono, the intermediate backbone plasmid for expression vectors bearing antibiotic resistance genes was obtained by removal of the region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector.

Here we demonstrate that mtr-miR159b is effectively processed from its precursor molecule and thus represents a highly suitable backbone for the expression of amiRs in M. truncatula.

TFAAA-USP18 in the pACE vector backbone was tested for expression in E. coli BL21 (DE3) and E. coli BL21 (DE3) pLysS.

To this end, we are developing additional Flp-in vector backbones for inducible expression of shRNAs to allow for specific knockdown of target genes.

miRNAs expressed and processed at high levels in the ovary and early embryo are good candidates to function as backbones for toxin expression in both systems (Table S18 and Table S19).

The pRS426-GD plasmid was constructed with GAPDH 5'-promoter (upstream 500 bp) with/without an alpha factor sequence and 3'-terminator region (downstream 300 bp) of the S. cerevisiae genomic sequence into the pRS426 plasmid backbone for cellulase gene expression (Additional file 6).

In addition, because the expression vector backbone contains a dual promoter system for expression in bacterial and mammalian cells, the final construct can be used directly in cell imaging studies.

Other enzymes required to decorate or modify the diarylheptanoid and gingerol-related backbone structures could well belong to the other major gene families evaluated for expression.

However, our profiling of CK1δ expression provides an anatomical backbone for future studies targeting cell-type specific functions of CK1δ in various tissues and organs.