Sentence examples for backbone for construction of from inspiring English sources
pBSXH, which comprises the hph-marker cassette [ 34], served as backbone for construction of the deletion vectors described in the following.
Reassortment of animal rotavirus segments onto human strain backbones has been documented (28 ), and experimental insertion of human rotavirus strains onto animal strain backbones was the basis for construction of reassortant rotavirus vaccines Rotashield and RotaTeq (29 ).
The backbone for the construction of the comorbidity index was the National Discharge Registry.
The pBlueScriptII SK (Stratagene) was used as a backbone for the construction of the autonomously replicating plasmids.
The Ad5 backbone vector pAdEasy(E3) and the transfer vector pShuttle-i/CgA-E1A, which were used for construction of Ad[CgA-E1A] have been described earlier [11], [19] and so has the constructions of AdMock and Ad[CMV-Luc] [20] and the prostate-specific Ad[iPPT-E1A] [19].
Specific protocols for construction of these AAV vector scaffolds, shuttle cloning of their contents into AAV and lentiviral backbones, and a quantitative luciferase assay capable of screening for optimal sgRNAs, are detailed.
For construction of the Halobacterium sp. pARK and pDRK expression plasmids, pMC2 expression plasmid was used as the backbone [ 26, 27].
For construction of the suicide plasmid pM2401, a sopEM45-fragment (lacking the N-terminus) was PCR-amplified from genomic DNA of SB875 [25] using the forward primer 5'-CAGACGTCAACAGGAAACCACACTAC-3' and the reverse primer 5'-CTGGATCCGCGTCTCTGTC-3'. The resulting PCR product was then cloned into the AatII- and BamHI-digested backbone of pM1132 [26] carrying the tem-1 gene.
For construction of Tg (lmo2 kri1l) transient transgenesis plasmid, the zebrafish lmo2 promoter was obtained by PCR amplification from lmo2-Cre-PBSK-I-SceI plasmid, and then cloned into the Tol2 transposon backbone together with full-length kri1l gene.
