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pBSXH, which comprises the hph-marker cassette [ 34], served as backbone for construction of the deletion vectors described in the following.
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The backbone for the construction of the comorbidity index was the National Discharge Registry.
The pBlueScriptII SK (Stratagene) was used as a backbone for the construction of the autonomously replicating plasmids.
The vectors pEU3-NII (Invitrotech) and pEU-E01 (Cell Free Sciences were used as backbones for plasmid construction.
The obtained nanosheets array has been directly employed as 2D backbone for the subsequent construction of hybrid nanostructure of Co3O4@NiMoO4 by a simple hydrothermal synthesis.
Reassortment of animal rotavirus segments onto human strain backbones has been documented (28 ), and experimental insertion of human rotavirus strains onto animal strain backbones was the basis for construction of reassortant rotavirus vaccines Rotashield and RotaTeq (29 ).
The size of the binary vector by agro-infiltration is usually large and its backbone has numerous restriction sites that increase difficulty for construction.
The Ad5 backbone vector pAdEasy(E3) and the transfer vector pShuttle-i/CgA-E1A, which were used for construction of Ad[CgA-E1A] have been described earlier [11], [19] and so has the constructions of AdMock and Ad[CMV-Luc] [20] and the prostate-specific Ad[iPPT-E1A] [19].
Specific protocols for construction of these AAV vector scaffolds, shuttle cloning of their contents into AAV and lentiviral backbones, and a quantitative luciferase assay capable of screening for optimal sgRNAs, are detailed.
For construction of the Halobacterium sp. pARK and pDRK expression plasmids, pMC2 expression plasmid was used as the backbone [ 26, 27].
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