15N relaxation parameters, T1, T2 and 15N-{1H} steady-state NOE were measured for twelve backbone environments at two spectrometer frequencies, 500 and 800 MHz.
The absence of NMR CSPs for the positively charged residues in ZnF1 upon RNA titration (Fig. 6A) is likely due to similar chemical environment for backbone amide protons when surrounded by water or interacting with RNA, and is also indicative that the interactions between ZnF1 and RNA is mainly electrostatic and likely nonspecific.
The primary use of only one type of structural lipid may have been driven in part by the difficulties of reconciling metabolic pathways that use different chiral forms of the lipid glycerol backbone, with the mesophilic environment removing any intrinsic benefit of ether-linked lipids.
Data centers, which are the backbone of corporate computing environments, now need far fewer physical machines.
Internet Protocol (IP) over Wavelength Division Multiplexed (WDM) networking technology with Terabits per second bandwidth become a natural choice for the future generation internet networks (Optical Internets), wide area network (WAN) environments and backbone networks due to its potential ability to meet rising demands of high bandwidth and low latency communication.
Since the PIPn ligand motifs used in this analysis lack the membrane environment and glycerolipid backbone, yet still exhibit high-affinity protein binding, these results narrow down the structural requirements for Akt recognition.
Despite its low hydrophobicity, the P56S major sperm protein (MSP) domain becomes largely embedded in the membrane environment with high backbone rigidity.
T108R1 appears to be slightly more immobile than N151R1 in the native state, which could be due to differences in backbone dynamics or the surrounding environment.
Astonishingly, the P56S MSP domain is mostly embedded in the membrane environment with high backbone rigidity, and is composed of five well-formed helices at N- and C-ends linked by a long unstructured loop.
