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Accurate quantification of the 15N {1H} steady-state NOE is central to current methods for the elucidation of protein backbone dynamics on the fast, sub-nanosecond time scale.
The NMR relaxation parameters extracted for all twelve environments are consistent with MA silk protein backbone dynamics on the fast sub-nanosecond timescale.
We have also measured the H-N hetNOEs of C72S at pH 6.0 and pH 3.0 to probe fast backbone dynamics on the femtosecond to picosecond timescale [ 36].
The NLS folds onto precisely those residues in the well-structured part of IκBα that undergo backbone dynamics on the microsecond to millisecond time scale in the free protein.
In addition to structural information, the EPR lineshapes of R1 at solvent-exposed sites on helices also reveal the amplitude of local backbone dynamics on the nanosecond time scale.
DOI: http://dx.doi.org/10.7554/eLife.07888.014 > > -wrap-foot> To further characterize differences in structure and dynamics of PLK4-PB3 seen upon STIL-CC binding in aqueous solution, free and STIL-CC-bound PLK4-PB3 were subjected to 2D [N,H]-TROSY experiments to reveal chemical shift perturbations and to N-{H}NOE measurements to characterize backbone dynamics on the ps- to ns-timescale.
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Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues.
Such modification is likely to alter backbone dynamics of the protein.
To analyze the backbone dynamics of the I214V mutant of RaPrPC(121 228), we performed 15N relaxation measurements using NMR spectroscopy.
Having established FRAGFOLD-IDP and taking advantage of this separation, we can now compare whether the quality of backbone dynamics predictions depend on the quality of structure predictions.
Lack of NOEs and rapid NH exchange for L53AI54A, combined with detailed analysis of the backbone dynamics, indicated that the hydrophobic core of this variant is not uniquely structured, but fluctuates on the NMR timescale.
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