Sentence examples for backbone downstream of the from inspiring English sources

The cDNA was cloned between the LTRs of the lentiviral backbone downstream of the ubiquitous active spleen focus-forming virus (SFFV) promoter.

They differ in the antibiotic resistance that is used for their selection in mycobacteria (kanamycin resistance or streptomycin resistance) and the qTags that are located in the plasmid backbone downstream of the target gene.

The α-helical 8 kDa lyase domain includes a structural motif that binds a monovalent metal interacting with the DNA backbone downstream of the single-nucleotide gap.

The terpenoid backbone downstream pathway is responsible for biosynthesis of geranyl diphosphate (GPP), farsenyl diphosphate (FPP), and geranylgeranyl diphosphate (GGPP), which are the precursors of monoterpenoids (C10), sesquiterpenoids (C15), and diterpenoids (C20), respectively.

SMURF parameter d (maximum intergenic distance in base pairs) was set at 3000 bp, and SMURF parameter y (the maximum number of non-secondary metabolism genes upstream or downstream of the backbone gene) was set at 6. Clusters of orthologous genes among the fungal genomes were identified using OrthoMCL version 2.0.9 [ 160] using an inflation factor of 1.5.

For colony-PCR after E.coli transformation the nested PCR forward primer was used in combination with a reverse primer designed to anneal 200 bp downstream of the ORF to the vector backbone (Fig. 6b).

For the baculovirus insect cell expression system, a modified pFastBac Dual vector backbone with the melittin secretion signal sequence (Tessier et al. 1991) and an N-terminal HIS-tag encoded downstream of the polyhedrin promoter was used as expression backbone.

For the Mis2r-Hsp68-lacZ construct, a 0.9 kb fragment of the Hsp68 minimal promoter was also placed downstream of the Mis2r promoter in a Bluescript KS vector backbone (Brugger et al., 2004).

Putative miR-181b MREs containing synthetic sequences were cloned into Spe I and Hind III sites in the multiple cloning region downstream of the firefly luciferase gene in pMIR-REPORT (Ambion) backbone as described [ 27, 28, 71].

These sequences were then inserted upstream and downstream of the GFP or RFP sequences in the respective p417cyc backbone vector.

We designed gammaretroviral and lentiviral vectors that contained identical enhancer promoter sequences located either in the LTR or downstream of the packaging region, for internal initiation of transcription from SIN backbones.