Sentence examples for backbone construct from inspiring English sources

shRNAs against human lncRNAs were obtained by applying the SENSOR design rules61 and subcloning the 97mer oligos into a pLKO5d.SFFV.eGFP.miR30n backbone construct (Addgene #90333).

Most likely, the thick main-chains of PPABCS obtained by "jacketing" the central rigid portion of side-chain to the polyethylene backbone construct a 2D centered rectangular scaffold.

Pseudotyped viral particles of HCV or VSV were generated by co-transfection of the JFH1 full-length envelope-expressing plasmid (pCDN3.1-JFH1-cE1E2) or the vesicular stomatitis virus glycoprotein (pCDN3.1-VSVG) together with the pNL4-3R-E-luciferase backbone construct into HEK 293T cells as described previously (Cui et al., 2013; Shukla et al., 2010).

GST ZF123 EGFP was chosen as the backbone construct for the substitution analysis (Fig.  5A), because a ZF1 mutation affects the nuclear localization.

Four types of CAG repeats encoding Q11, Q13, Q28, and Q165 were cloned into this backbone construct using two KpnI sites flanking the CAG repeat sequence, producing the final rCTF-Q11, rCTF-Q13, rCTF-Q28, and rCTF-Q165 cDNA clones.

After checking its internal nucleotide (nt) sequence by DNA sequencing, this clone was digested with BamHI and BglII (corresponding to the nt number 5861 in AB035727), and the resultant fragment was cloned into the BamHI/ BglII site of Clone A, producing the backbone construct for the recombinant HA-CTF-myc fusion protein.

Integrating nanostructured MoS2 with low-volume-change and high-rate-performance TiO2 backbone to construct high structure stability MoS2-TiO2 based composites has been turned out to be an effective strategy.

PPARγ-null TS cells were infected with the highly purified adenovirus; virus expressing only the backbone GFP construct, without the PPARγ1 gene, was used as control.

LentiORF RFP-expressing viral particles (from the same backbone pLOC construct) were used as a control.

pGL4.11 (Promega) carrying the luc2P reporter gene was used as a starting backbone to construct the combinatorial transgene expression cassettes through multistep subcloning.

The pSilencer™4.1-CMV neo and pSicoR-CD14 shRNA-674 plasmids were first digested with BamHI and HindIII, respectively, then the CD14 shRNA-674 fragment was inserted into the pSilencer™4.1-CMV neo backbone to construct the pSilencer™4.1-CD14 pSilencer™4.1-CD14