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For the crystalline phase, a periodicity in the plane of the surface of 4.15 Å is observed corresponding to half the fluorene ring repeat distance along the backbone, consistent with interdigitating side-chains.
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In all cases examined, the unwinding and rewinding process of the helical backbone is consistent with the zippering mechanism previously elucidated for the concentrated LC solution.
The limited number of mutations observed in the genome backbone are consistent with mutation rates in bacteria previously calculated using data from both experimental (e.g., [ 2, 3]) and natural populations [ 38].
In addition, it is reasonable to propose that C2 symmetric molecules such as 1 should have a distinct advantage when it comes to association rate constants, because overall C2 symmetry ensures that all initial encounters with DNA occur with a backbone orientation consistent with formation of a bound complex.
The predicted conservation of backbone flexibility is consistent with natural expectations.
It is worth mentioning that our backbone flexibility measures are consistent with those from Elastic Network Models, which are sensitive to fold, but not sequence.
The directions of the chemical shift changes and small changes in backbone JHH couplings are consistent with an increase in the α-helicity of the first six residues of aS, although a high degree of dynamic conformational disorder remains and the helical structure is sampled <20% of the time.
A pronounced decrease of the higher energy REP component is apparent indicating conversion to PBTTT chains with improved backbone and side group order consistent with intercalated bimolecular crystals.
This stabilization of helical structure is accompanied by an increase in the heteronuclear NOE, consistent with decreased backbone flexibility due to enhanced packing onto the molten globule core.
Moreover, the highest occupied molecular orbital (HOMO) energy level had been obviously lowered, consistent with the suitable backbone twists in main-chains.
We decided to take advantage of this property to generate a large number of amino acid sequences consistent with the 3D backbone of known proteins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com