Sentence examples for backbone assignment from inspiring English sources

Here, we report purification and NMR backbone assignment of recombinant Grx2 proteins.

Previously, we reported the backbone assignment result of Cys version Grx1 (Lee et al. 2012).

Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here.

The methods utilised to achieve the backbone assignment are described in detail, including a semi-automated protocol based on a simulated annealing Monte Carlo technique.

All NMR data were processed and analyzed by TopSpin (Bruker BioSpin), NMRPipe (Delaglio et al. 1995) and then applied to AutoAssign server (Zimmerman et al. 1997) and further backbone assignment was performed by Sparky (Goddard & Kneller 2004) software packages.

For very large proteins in the highest magnetic fields, the large chemical shift anisotropy (CSA) of carbonyl carbon deteriorates coherence transfer efficiency in experiments designed for unambiguous sequential backbone assignment.

The high degree of spectra overlap made backbone assignments difficult for this domain, yielding 60%% of the resonances assigned for p12.

Their subsequent elimination resulted in a reduced-size binder with very similar affinity for NT, for which near-complete backbone assignments were achieved.

Previously published backbone assignments [28] were used in this study.

However, backbone assignments were made for 95%% of the non-proline residues of CAN.

Sequence specific backbone assignments were made using 3D HNCO, HNCA, HN CO CA, HNCACB and CBCA CO NH data.