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Sequence extraction, realignment of each conserved segment (backbone) and SNP determination were all performed as described above (see determination of SNPs section).
He has guts and determination.
Triples take grit and determination.
These results confirm the well-established backbone and the sub-haplogroup determination of the currently used Y chromosomal phylogenies.
One of the challenges has been to automate the final steps beyond backbone and sidechain peak assignment, including the determination of torsion angle constraints, the assignment of NOESY cross peaks and the determination of distance constraints, the analysis of secondary structure, and the calculation of a validated 3D protein structure.
We have developed a suite of novel polynomial-time algorithms, called RDC-ANALYTIC [1, 3, 4], for high-resolution protein backbone structure determination from a minimal amount of residual dipolar coupling (RDC) and residual chemical shift anisotropy (RCSA) data.
Algorithms and Analytic Solutions using Sparse Residual Dipolar Couplings for High-Resolution Automated Protein Backbone Structure Determination by NMR. A. Yershova,* C. Tripathy,* P. Zhou, and B. R. Donald.
Just will and determination.
Timing, and determination.
Discipline and determination.
Our sincerity and determination would be questioned.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com