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Interestingly, the extract from the double knockout cells was essentially devoid of in vitro BER, indicating a modest back up role of another polymerase in the absence of pol β (Fig. 2A, lanes 10 12; Fig. 2B, lanes 10 12).
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Thus, B-NHEJ does not seem to play a major role in the repair of our cassette, however, due to the incomplete elimination of NHEJ when available XRCC4 was knocked down, we cannot rule out that B-NHEJ is serving a back-up role which would only reveal itself when XRCC4/Ligase IV activity is completely eliminated.
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