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GC content, read length and damage all had significant impacts on the proportion of read pools able to be mapped back to their reference at both levels of divergence, while coverage depth was not significantly correlated for either low or high divergence read pool mapping (Table 1).
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The resulting dataset of reads (raw reads, trimmed reads and merged reads) were all mapped back to their corresponding reference genomes with BWA-MEM using default parameters [ 35].
These pools were subsequently mapped back to their corresponding reference sequences using commonly applied alignment software and at a range of ultra-low to moderate sequencing coverage depths (0.1×, 0.5×, 1×, 2×, 4×, 8× and 16×).
Ms Cooper's intervention comes in stark contrast to her Labour parliamentary colleague, Keith Vaz, who told the BBC today that the solution would be to 'deport them back to their countries', referencing the thousands of immigrants attempting to make their way to the United Kingdom.
However, a major technical hurdle lies in the need to map short sequence reads back to their correct locations in a reference genome.
Altogether, the longer reads generated with the FLX System greatly facilitated their mapping back to the reference genome sequence.
Concerning specifically repeat expansions, they cannot be mapped back to a reference genome unambiguously due to their repetitive nature.
Most of the fragments are normal, and their paired reads map back to the reference genome about 500 bp apart and in the correct orientation.
These resulting sequences are then mapped back to a reference genome to determine the position of their origin.
After mapping the sequenced reads back to a reference genome CAGE data highlights the transcriptional start sites (TSSs) and their usage at a single nucleotide resolution.
Meaning that it will be there in your 'Favorite Bag' to go back to and reference any time you like.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com