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2.4.3 brings us back to orientation maps.
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Carney says this goes back to the orientation of the businesses, and the sense of resonancy.
From the structures observed for phosphorylated yeast Ire1, the protein must rearrange on autophosphorylation from the face-to-face orientation to the back-to-back orientation that is suggested to be the state of the protein required for RNase activity.
This orientation is distinct from the 'RNase active' back-to-back orientation that has been seen in all the yeast structures thus far, and represents a 'phosphoryl-transfer' competent state.
Here, a double-stranded oligonucleotide consisting of two primer sequences in back-to-back orientation was designed as an adapter for LM-PCR.
Back-to-school orientation for teachers now includes safety drills on how to respond to a gunman.
The other night as I reviewed back-to-school orientation dates, I reminded myself that beneath the emails and accommodations is the child for whom I am advocating.
Whether the back-to-back orientation exists in the human Ire1 mechanism remains to be seen.
In contrast, dimerized Sc Ire1 RNase domain holds a back-to-back orientation when activated.
More recently, a yeast Ire1 quercetin ADP complex (Wiseman et al, 2010) showed essentially the same back-to-back orientation that has been seen with the previous structures.
The genes encoding the subunits (Fer1HCH, Fer2LCH) are positioned in the Drosophila genome in a back-to-back orientation, enabling coordinated regulation of their transcription [ 55].
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