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Patterns of past gene duplication defined as back to back copies of genes that share nucleotide sequence similarity and retail the same functional annotation occurred frequently within regions of CNV (supplementary table S1, Supplementary Material online; regions of similarity are colored).
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The dual constitutive control element consists of back-to-back copies of a baculovirus immediate early (ie1) promoter separated by a baculovirus enhancer (hr5).
The dual inducible control element consists of back-to-back copies of a minimal cytomegalovirus (CMV min ) promoter separated by a synthetic operator (TetO7), which drives transcription in the presence of a mutant transcriptional repressor plus tetracycline.
Subsequently, the P CMVmin fragment was excised from pCR2.1-HpaCMVNru with HpaI and BamHI and subcloned into the corresponding sites of pCR2.1-PmeTetO to produce pCR2.1-NruTetOPme, which contained the fully assembled control element consisting of back-to-back copies of the CMV promoter separated by the tetracycline-inducible operator.
Therefore, we constructed a second new set of piggyBac vectors in which pairs of heterologous genes could be placed under the control of a mosaic, tetracycline-inducible control element, which consisted of two back-to-back copies of the minimal human cytomegalovirus immediate early gene promoter (P CMVmin ) separated by an operator (TetO7).
Back to Back".
"It's back to back to back".
Back to back to back.
Belly to belly and back to back!
It's a back to back!
I see patients back to back.
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