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She turns back to face the large stainless stock pot that is resting atop a folding table and gets right back to assembling another plate.
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Then the reads are mapped back to assembled contigs.
Raw reads were mapped back to assembled contigs using BWA [ 48].
The read pair classification is based on mapping of MP data back to assembled contigs from PE data.
Clean reads were mapped back to assembled unigenes with SOAPaligner (version 2.21) [ 44] allowing maximum 2 mismatches.
In addition to using results from blast against other model species, one can also detect chimeras by mapping reads back to assembled transcripts to identify areas with a sudden change in read coverage.
The cleaned reads of each sample were mapped back to assembled contigs by bowtie2 with the following parameters: maximum mismatches in seed alignment = 1; length of seed substrings = 22.
Exceptionally high levels of heterozygosity (0.85 % of single nucleotide polymorphisms plus sequence insertions or deletions) complicated assembly; nevertheless, 96.4%% of reads mapped back to the assembled scaffolds, indicating that the assembly included most of the sequenced genome.
After assembling, reads were also mapped back to the assembled transcripts with a length ≥ 100 bp.
Subsequently, reads were mapped back to the assembled contigs to improve assembly using bowtie.
After three albums proper, Melbourne's cult garage rockers rifle through their wonderfully idiosyncratic back catalogue to assemble this odds'n'sods package of singles, alternate takes and compilation tracks.
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