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Its crowning monument is the Palazzo della Civiltà Italiana, a stripped back stack of arched colonnades that stands at the end of a grand axis like a cubic colosseum.
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On a more positive note, at least for die-hard Delicious del.icio.us fans, Aly also promises to bring back Stacks.
Either way, within the next 10 days Australia's cricketers will be going home for the second time in two years and the current back-to-back-back triple stack of conjoined Ashes tours will finally be over, a 15-match gorge that has felt at times less like a celebration of cricket, more like the end of something.
Together, these findings indicate that Gln1 assembles into filaments by a back-to-back stacking mechanism.
10.7554/eLife.02409.011 Figure 2. Gln1 assembles by a back-to-back stacking mechanism.
This indicates that the structure of Gln1 remains intact and that assembly proceeds by the proposed back-to-back stacking mechanism.
This may imply that the mutations in general are more detrimental to how actin functions as a filament than as the monomer, and that between stacking and backing, stacking has more influence over the filament stability.
They go on to suggest that Gln1 filaments form by back-to-back stacking of native or near-native structures and show that filament formation is important for successful recovery from starvation.
So while we agree with the reviewer that the pH-induced back-to-back stacking interactions may actually be quite stable, additional interactions – in particular those between individual filaments – may be rather weak.
We demonstrate that starvation induces the self-assembly of yeast glutamine synthetase into filaments by a simple back-to-back stacking mechanism (see Figure 7B for a model of assembly).
Filament formation by Gln1 is a highly cooperative process, strongly dependent on macromolecular crowding, and involves back-to-back stacking of cylindrical homo-decamers into filaments that associate laterally to form higher order fibrils.
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