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Consecutively, four round full-thickness excisions, 4 mm in diameter, were performed on the back of each rat.
The back of each rat was shaved with electrical clippers and then cleansed with three cycles of a wash and rinse with betadine and alcohol, respectively.
Wounds (1 cm diameter/each wound) were created on the back of each rat, and three pieces of cell-Matrigel were placed in three distinct wounds on each side of the rat's back.
Under general anesthesia with intraperitoneal injection of sodium pentobarbital (30 mg/kg), an osmotic pump was implanted under the skin on the back of each rat (Galatz et al. 2006).
For the investigation of topical application of the drug to the tumour tissue, and for the experiment measuring TIFP using the wick-in-needle method, the tumour cell suspension was injected s.c. into the back of each rat.
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The next day, full thickness wounds of 8 mm diameter were inflicted on the back side of each rat using a special type of sterile circular blade, Acu-Punch (Acuderm Inc., FL, USA), under light ether anesthesia.
The acceleration sensor module was attached directly to the shaved back of the rat's body to directly measure the animal's vibration.
Hairs on the back of a rat take three weeks to grow fully, whereas the follicles on the human scalp may be continuously active for three years or more.
The longer, thicker whiskers at the back of a rat's face, they found, resonate at lower frequencies than the shorter, finer feelers near its mouth.
The pump was placed subcutaneously on the back of the rat.
The osmotic pump was connected to the brain kit and placed at the back of the rat subcutaneously.
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