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The most powerful medical scientist in Britain today reiterated his threat to quit if ministers failed to back experiments on animals.
A small group of leading climate scientists, financially supported by billionaires including Bill Gates, are lobbying governments and international bodies to back experiments into manipulating the climate on a global scale to avoid catastrophic climate change.
We performed depletion/add back experiments and studied the effect of the replacement of endogenous Eg5 by mutated Eg5 on spindle assembly (Fig. 3B).
For proteins used for add back experiments, the β-mercaptoethanol was omitted from the dialysis buffer, which was instead degassed, and the dialysis lasted only for 3 h.
For depletion/add back experiments, 80 µl of protein A Dynabeads coupled to anti-Eg5 antibody, or in controls an irrelevant IgG ('mock' depletion), were used to deplete 100 µl extract for 1 h on ice.
This raises the possibility that the "add back" experiments are subject to an in vitro artifact.
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The latest running back experiment included Maurice Clarett, who was waived.
One choice is permitting federally backed experiments on a handful of cell types, called lines, that have already been identified, but banning public money to develop more.
Open image in new window Fig. 5 Fracture obtained from mine-back experiments and its matching patterns.
Huang and Kim (1993) conducted mine-back experiments, which show that a hydrofracture does not propagate linearly, but there are bi-wing fractures with several branches.
Pyrosequencing results show the assay to be extremely (>99%) sensitive, and spike-back experiments demonstrated a detection threshold of <102 CFU mL−1.
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