Sentence examples for back assay from inspiring English sources
To ensure that the selective inhibition of TRAIL-induced death without affecting survival in response to cytotoxic chemotherapy affected long-term growth, we performed a cell grow back assay by treating cells for 24 hours with TRAIL or etoposide then washing out the drug and allowing any surviving cells to grow back.
The second assay (Back Assay as it leads to NADPH formation) is based on the FabG-catalyzed oxidation of 3-OH decanoyl-NAC with NADP+ as cosubstrate.
In the Back Assay, the KM of 3-OH decanoyl-NAC could not be determined due to limited solubility of the substrate.
Limits on compound availability and a pragmatic raise of the cutoff to 50% resulted in 30 compounds being selected for concentration/effect curves in the Back Assay.
The FabG high-throughput screen was performed using the Back Assay and a library of 15667 compounds (Dundee Drug Discovery Unit in-house diverse compound collection).
FabG was screened against a diverse library of 15667 compounds at a single concentration (10 μM) using the Back Assay with 3-OH decanoyl-NAC as substrate.
As a final test of the inverted structure of the amplicons, we carried out snap-back assays (Tanaka et al. 2005).
Only 2 patients of the 14 with CMV IRU who were tested exhibited unusually robust suppression, which was confirmed by add-back assays to be caused by CD3+CD4+CD25+CD127low cells (data not shown).
For snap-back assays, NIB-and-Grab DNA was digested with either ApaLI or EcoNI, denatured at 100° for 10 min, chilled immediately on ice for 7 min, and then ethanol precipitated and resuspended in 8 μL of H2O.
High levels of CO2 induced Na,K-ATPase α1-subunit phosphorylation as assessed by a "back phosphorylation" assay (Figure 6A).
The back-mutation assay method for determining the mutagenicity of various treatments was first developed a little over 50 years ago and has been in continuous use ever since.
