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Y. pestis bacilli were observed by light microscopy using a Nikon Eclipse E200 microscope at a magnification of ×1000.
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Small extra- and intracellular bacilli were observed directly inside the shell vial by using Gimenez and Gram staining.
In the guinea pig model, most bacilli were observed within the necrotic regions of the primary granulomas by IF and AR staining.
No organisms other than acid-fast bacilli were observed.
In samples from stationary phase culture, two populations of green and yellow bacilli were observed.
Large numbers of bacilli were observed, none with the morphologic features of anthrax.
In lesions of 51 animals (47% of animals with lesions; CI: 38%–57%), acid-fast bacilli (AFB) were observed by direct microscopy (Table 1).
In the unorganized lesion structure of the GKO mice, a very rapid clearance of AR+ bacilli was observed, presumably by the large number of phagocytic cells and macrophages present.
Many bacilli suggestive of Bartonella spp. were observed by Warthin-Starry staining, and antibodies against B. henselae (titer 256) were detected in serum.
In addition to the single stained bacilli another subpopulation of dual stained yellow bacilli was observed which was a smaller population than either green or red.
The finding of male sex and of ischemic heart disease being risk factors for carriage of and infection with resistant gram-negative bacilli had also been observed by our group, as well as by others for carriage of extended spectrum β-lactamase (ESBL ‑producing Enterobacteriaceae and Pseudomonas aeruginosa (29 – 31 ).
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