Sentence examples for bac into from inspiring English sources

It is possible to subclone the entire insert of the BAC into a modified BAC vector [ 10, 11] however, this involves cloning >100-kb fragments which is technically tricky.

(1) It is possible to cluster the short reads obtained from each BAC into small local sets that represent the sequence of one BAC where most of the repeat sequences have a unique copy.

In general, a bacterial artificial chromosome (BAC) harboring most regulatory elements is capable of overcoming the limitations, but transferring BAC into donor cells is difficult.

"En passant" recombineering is applied to the insertion of a transgene expression cassette and the removal of the selectable marker in bacteria; and a self-excising variant of MVA BAC is constructed, in which the BAC cassette region is rapidly and efficiently lost from the viral genome following rescue of the BAC into infectious virus.

Release of BAC into water medium from prepared composites were shown to depend on the synthetic method and differed significantly.

Recently it has been reported that the introduction of BAC into human tears hinders the ability of native lipids to spread across the tear films and can result in the replacement of those lipid with BAC molecules [17].

For BAC by BAC sequencing, tiling BACs into contiguous linear arrays is usually achieved by high throughput fingerprinting [6], [7].

Unlike earlier constructs, the new CHV-BAC allowed self-excision of the bacterial genes via homologous recombination after transfection of BACs into cell culture.

Transgenic mice were generated at the Karolinska Center for Transgene Technologies (Stockholm, Sweden) by injection of NotI-linearized rBDNF-lacZ-BAC into CBA x C57Bl/6 mouse pronuclei.

We classified the BACs into one of four types, according to the pattern of BLAST hit.

We describe a method for linking any two overlapping BACs into a single clone.