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15% SDS-PAGE of WT Ec2 (A ) or Bpe (B ) and indicated lysine mutants treated with 0.125% glutaraldehyde for the indicated times.
Indirect immunofluorescence microscopy studies clearly confirmed the glycosylation studies (Fig. 2A, B) and indicated that the six-transmembrane domain topological models of both FIT1 and FIT2 were correct, in which both proteins have their N- and C-termini facing the cytosol (Fig. 1A, C).
(C ) The fluorescence changes in GCaMP7a (ΔF/F0) of the ECs indicated by arrowheads in B and indicated at the left panel are shown as a graph.
Visual predictive checks of the separate covariate effects identified in the final covariate survival model are depicted in Figure 3 B and indicated adequate description of the empirical survival curves.
As shown in Figure 2 F a and b, and indicated by the asterisk, there are cells exhibiting a high level of green fluorescence (specifically in the nucleus) but which do not colocalize with the red fluorescence signal of the anti-CD133 antibody.
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Colors correspond to the wiring diagram (B ) and indicate which neuronal class expressed csChrimson.
Arrowhead in (B and B' ) indicates a nasal cavity cell likely about to ingress that has increased Sox10 protein expression.
In the case of SeCitS, comparison of the inward-facing protomers B and B' indicates unambiguously that citrate is released before Na+, and that Na2 is released before Na1.
The Hosmer and Lemeshow tests for Models B and C indicated a lack of fit.
A and C indicate low-magnification, B and D indicate high-magnification.
Arrows in B and D indicate co-expression, arrowheads indicate lack of co-expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com