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Alterations in the strain magnitudes along the mesio-distal axis were quantified for each stage and compared with the Mechanostat predefined regions.
For P7 and embryonic cortical cell quantifications, 200 µm-wide columns perpendicular to the lateral cortical axis were quantified for immunopositive cells in equivalent areas from three adjacent cryosections per brain (n = 3 5).
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Following previous work [ 47, 84, 85], degrees of growth around the columnellar axis was quantified.
To investigate this, the minimum separation of large (>10 RyR) clusters in the longitudinal axis was quantified.
The amount of movement in the dorso-ventral axis was quantified using a supervoxel based Optical Flow algorithm (Amat et al., 2013).
The effect of lamin A/C down-regulation on nuclear size (diameter of the long axis) was quantified by computer-assisted imaging analysis, and the results indicated that lamin A/C down-regulation caused a general increase in nuclear size.
Pixel intensities of red and green CKC signals in native chromosomes released from prometa- or metaphase embryos were quantified along the axis connecting the two sister kinetochores (Fig. 7a c).
Cell morphology changes were quantified as ratio of long axis to short axis for each cell.
Cell morphological changes were quantified as a ratio of long axis to short axis for each cell.
The mitochondria in the cells of developing endosperm were quantified along a dorso-ventral axis (the lateral regions of the endosperm are not included in this axis).
Intraocular distances were quantified dynamically along the central axis of the eye.
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