We show that two axes of gene expression co-variation, each including hundreds of transcripts related to lymphocyte and neutrophil activity, are differentially expressed during AMI.
It is likely that the very high covariance of gene expression into axes of coordinated gene expression that explain over half of the variance among individuals readily induces correlations that do not replicate.
To identify major axes of variation in testis expression, we performed a principal components analysis using prcomp in R (R Development Core Team 2010) with scaled variables.
Two aspects of personal transcriptomes are particularly noteworthy: the coordinate regulation along conserved axes of covariance, and the nature of expression at the extremes.
However, it appears that genes having similar and/or coordinated expression are often clustered [ 5]: the genes comprising the vertebrate β-globin cluster are organised according to the timing of their expression during development [ 6] with members of the mammalian homeobox transcriptional regulator loci arranged according to their spatial pattern of expression along developmental axes [ 7].
The five axes of sustainable development are expressions of: prosperity, equality, and governance; quality of life; ecosystem integrity; environmentally efficient happiness; and environmental management.
Examination of expression patterns implied these miRNAs have roles in patterning the anterior-posterior and dorso-ventral axes as well as the extraembryonic membranes.
The resulting lists of genes, ordered by function, were plotted on both axes of a matrix containing all pairwise gene expression profile correlations.
To explore the nature of the differential expression further, we evaluated the nine axes of variation that we recently showed are highly conserved in peripheral blood gene expression profiles from multiple studies in healthy and disease cohorts [ 17].
In HistoQuest software (TissueGnostics, Tarzana, CA, USA), we plotted the X and Y axes for the expression of the indicated proteins.
