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This procedure allowed to obtain a frequency tuning curve at once for a given parameter setting, avoiding variation of the frequency of a sinusoidal force.
To carry out the process as identically as possible for the three time points, we barcoded the cell samples allowing staining procedure for all three samples in the same tube and thereby avoiding variation between sample time points.
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All subjects had a normal body mass index (BMI), thus avoiding variations in the pharyngeal airways because of obesity.
Extreme care was taken to avoid variation in total biomass of the larvae in each replicated tanks and also for the entire experimental sets.
To avoid variation in DNA recovery, no further purification was performed prior to qPCR.
To avoid variation from responder T cells, we purified responder CD4+ T cells from one single healthy donor.
First, it standardizes the estimates of screening performance and avoids variation arising from the age range selected.
Average (over all loci) correlograms are presented to avoid variation in correlogram profiles based on the frequencies of individual alleles that are subject to stochastic processes.
A standard DNA extraction protocol was used for all samples to avoid variation in composition across sample type due to extraction method.
All the tasks were carry out in end expiratory, open glottis apnea in order to avoid variation in autonomic activity due to breathing.
To avoid variation due to circadian rhythm, blood was drawn in the morning between 8 and 10 am after a period of overnight fasting.
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