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To avoid genotyping errors, genotypes were scored independently by two investigators and checked for confirmation.
Two techniques were used to avoid genotyping errors.
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All adult individuals were genotyped twice (from PCR to genotyping) to prevent genotyping errors.
To detect genotyping errors, repeated genotyping of the same samples is preferable over HWE testing.
In the first step GenoType detects the genotyping errors and prepares an input file for GenoDive.
To avoid the identification of spurious recombination events due to genotyping errors, once a potential recombination has been identified for a particular individual, a temporary FMV is created to step through adjacent markers and validate the recombination event.
To avoid erroneously inferring non-Caucasian outlier status based on genotyping errors, we also examined each subject's mean BRLMM confidence score at all SNPs contributing to that subject's rare-het counts (column 7).
Therefore, the heterozygous genotypes were considered to be genotyping errors.
To prevent genotyping errors DNA samples were genotyped in duplicate by RealTime PCR based method.
Heterogametic individuals are phased de-facto, thus avoiding the possibility of phasing errors for these individuals, other than due to genotyping errors.
Progeny genotypes were further investigated for evidence of genotyping errors (error.prob < 0.01).
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