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However, we chose to establish fibroblasts stably expressing FAP to avoid fluctuation in the expression level of FAP under our experimental conditions.
To avoid fluctuation between individuals, total joint RNAs were pooled from three (TS) or five (KS) arthritic mice and five or six (WT) normal mice for replicates; poly (A)+ RNA was purified on oligo (dT -cellulose columns.
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As discussed above, in order to avoid fluctuations, the time interval should be set to a small value.
This reaction was conducted in between 11 am to 2 pm to avoid fluctuations in the intensity of sun rays.
Short testing times are necessary in a clinical context because they interfere minimally with routine care procedures and avoid fluctuations in vigilance, attention, and fatigue which can cause false negatives on ERP-based tests [42].
12 hrs day-night cycle was maintained to avoid fluctuations in the circadian rhythm.
PV systems require energy storage systems (batteries) to improve reliability and avoid fluctuations in power supply.
Samples were collected at the same hours in order to avoid fluctuations in gene expression.
A brief interval of 24 48 h was selected to avoid fluctuations in the severity of the symptoms.
HEPES was added at a final concentration of 30 mM to avoid fluctuations in pH while the measurements were accomplished.
To avoid fluctuations in the data caused by differences in the crystal size, we chose crystals similar in size.
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