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All experiments were duplicated in order to avoid experimental errors.
In order to avoid experimental errors arising from variation in the quantity and integrity of the RNA template, as well as the efficiency of the cDNA synthesis and PCR amplification, a normalization step is an essential pre-requisite.
Similar(58)
However, to avoid experimental deviations or the errors that inevitably occur during sample preparation procedures and data analysis, all of which make quantitation of gene transcripts unreliable, normalization of the qRT-PCR data is essential.
These precautions helped with taking precise readings to avoid the experimental errors suspected by Neuzil (Neuzil 1986).
This optical interrogation offers high accuracy and reliability compared with direct measurements of elongation, particularly at lower temperatures, thus avoiding significant experimental error.
We carried out all experiments at controlled R.T. and humidity (R.H. = 55 ± 5%) to avoid run-to-run experimental errors.
To avoid any significant errors resulting from the diverse signal intensities of the GeneChips (i. e., to minimize experimental errors), only array results with highly similar distributions of probe fluorescence intensities were included in the subsequent expression analysis.
These estimates indicate that most of the false positives predicted by PolyBayes were not experimental errors, but that the criteria used to assemble the ESTs and run PolyBayes were stringent enough to avoid a wide occurrence of paralogous SNPs.
This difference lies within the experimental errors.
Sources of experimental errors are outlined.
However, many of these values are associated with experimental errors.
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