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Cells are grown in T75 flasks and harvested during log phase to avoid contributions of quiescence to alterations in whole cell protein.
Recordings of tonic currents were performed at room temperature (20 24 °C) using the whole-cell patch clamp method to avoid contributions of Kv7 channels.
To test this, we measured for all genes the variation in splicing between K562 and GM12878 using cytosolic and nuclear RNA-Seq polyA+ data from ENCODE, using only junction-reads, to avoid contributions from RNAs stemming from overlapping enhancers (Methods).
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But if the level of contributions is high, it creates incentives for workers to become self-employed in what has come to be called the "black," or "underground," economy and for employers to avoid contribution liability by employing contract labour rather than full-time staff.
Laidlaw's government filings say Greyhound and the union are trying "to negotiate a method for avoiding contributions".
Claims by Brexit campaigners that the UK could enjoy continued membership of the single market while avoiding contributions to the EU budget are dishonest, three leading politicians have said.
These mechanisms aim to ensure smooth and relevant discussions, avoiding contributions that are inappropriate for their content (e.g., spam) or their form (e.g., interpersonal conflicts).
The court has forced a substantial amount of political speech underground, as contributors and candidates devise ever more elaborate methods of avoiding contribution limits, limits which take no account of rising campaign costs.
These credits are "mere accounting entries," he said, and by carrying them forward in this way companies can "avoid making contributions, often for years".
Ian Campbell Richmond upon Thames, Surrey The dearth of genuinely affordable homes to buy or to rent can only be made worse by the rule changes that allow property firms to reduce, or even avoid, making contributions to build affordable housing.
He adds: "...and there was a particular set of, I think, instructions given to the moderator to avoid certain contributions about a certain named individual who was a woman who, I think, who was a hoax victim.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com