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To avoid any variations in virulence, all pigs were challenged with bacteria from the same bacterial strain.
As for continuous catecholamine administration, this helps avoid any variations in delivery that may be induced by the injection of other drugs.
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The three-electrode system was always orientated in the same manner to avoid any variation from sample to sample.
Therefore, to avoid any variation due to the cell context, we evaluated these MAPKs in the same experimental system in terms of IM resistance.
The same protocol was used in both 2005 and 2008 to avoid any variation in methodology.
Forty-eight hours before the experiment, two subcultures were systematically performed to avoid any variation of the reference stain.
At 48 h before the experiment, two subcultures were systematically performed to avoid any variation of the reference strain.
For future studies, the automated application would be recommended to avoid any variation in application of treatment.
Therefore, to avoid any variation, step 3 was unified to be the combination of LABA with low dose inhaled steroids for both groups.
Counting was performed by an automated system (Varga et al, 2004) to avoid any variation possibly introduced by different observers and to eliminate imperfect reproducibility (coefficient of variation of the machine <5% for counts on the same slide).
Ideally the positive control should be provided by a central reference laboratory to avoid any variation attributed to differences in cell line expression of TH mRNA and sample preparation.
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