Exact(2)
Forty-eight hours before the experiment, two subcultures were systematically performed to avoid any variation of the reference stain.
At 48 h before the experiment, two subcultures were systematically performed to avoid any variation of the reference strain.
Similar(6)
For future studies, the automated application would be recommended to avoid any variation in application of treatment.
We employed three criteria for RSCA primer design: (i) complementarity to a conserved region, including all possible sequences in the detected MHC allele pool, (ii) amplification of most of the highly variable exon 2 (iii) binding of both primers within the exon 2 to avoid any length variation of the amplicon.
Samples were normalized internally using simultaneously the average cycle quantification (Cq) of Hypoxanthine PhosphoRibosyl-Transferase 1 (HPRT-1), Ribosomal Protein L 19 (RPL-19) and Hydroxymethylbilane synthase 2 (HMBS2) [75] as references in each sample to avoid any artefact of variation in the target gene.
Moreover, methods that seek to determine differentially methylated regions (Jaffe et al., 2012) also assume that probes within these regions are comparable and thus one would want to avoid any sources of technical variation within them.
These measurements were taken from the same spot on the sample to avoid any other sources of variation in signal.
To avoid any plate-to-plate variation of anti-CCP measurements, plates from the same batch (batch number 470094) were used; the inter-assay and intra-assay variabilities were less than 9%.
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