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But it allowed us to avoid alignments that are obviously suboptimal, and we could show that these class-3 alignments produce stable tree topologies.
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This measurement should avoid alignment mistakes between the polarization direction of the incoming light and the polarizer, since minor changes in the value of the TE polarization can significantly effect the extinction ratio.
Phylogenetic analyses excluded gap regions to avoid alignment ambiguities in regions with variation in sequence lengths.
To avoid alignment errors we retained only those with >60% identity at the protein level.
To avoid alignment efficiency biases caused by the presence of the insertion, the allele ratios were subsequently requantified using local realignment with the reference and mutated sequences.
The anchor length of the software was set at 8 nt and the mismatch number in these regions at 0 nt to avoid alignment bias.
Indeed, most WSP and other OMPs-based studies have excluded the HVRs to avoid alignment issues, although this resulted in exclusion of crucial functional domains of the protein.
In order to avoid alignment bias, qAlign will automatically inject the known single nucleotide variations into the reference genome to produce two new versions of that genome.
To avoid alignment artifacts and to come up with an optimal statement of homology, nucleotide sequences were visualized in Seaview 4.2.7 [ 51] and then converted to amino acid sequences for alignment with MAFFT [ 52] under L-INS-i algorithm, thereby preserving the open reading frame for these protein coding sequences.
First, DELLY uses affine gap penalties to avoid alignment regions interspersed with gaps and second, DELLY uses a global instead of a local alignment algorithm since the consensus sequence was derived from the read data and should thus be alignable across the full length.
To avoid alignment with too large divergence due to problematic ORF predictions from either species, we set a threshold for the percentage of identical amino acids to 50% and restricted the hits to contain less than 5 and 7 consecutive mismatches in Picea vs Pinus and Picea vs Taxus, respectively.
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CEO of Professional Science Editing for Scientists @ prosciediting.com