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(bottom) Projection averages were removed for clarity.
To ensure that correlations would not simply be due to differences between groups, group averages were removed from the data in this analysis.
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An alternative is to use deregressed EPD as the target variable, for which the parent averages are removed and appropriate weights can be applied to account for heteroscedastic residuals [ 33].
Points lying more than 1 standard deviation from the mean of a 121-point boxcar average were removed, as were additional points identified by hand.
Samples with TBP expression lower than 10% of the population average were removed from further analyses.
Before conducting the QTL analysis, outliers with a value greater than ± 3 s.d. of the trait average were removed to reduce the effect of strong deviation from normality.
One outlier with a standard deviation more than 12 times the average was removed.
A total of 96.4% of contributions due to records of internal information on average was removed from the joint information (Table 4).
Two of the progeny (untreated control 5 and spinosad survivor 12) had significantly fewer RAD reads than the average and were removed from the analysis, raising the average number of reads per individual to 461,500.
Briefly, spots with an average intensity, over all arrays, of lower than twofold above average background were removed from further analysis, then the intensities of the remaining 4,019 spots were normalised against reference.
For each breed, reads were trimmed at 33 bp and reads with homopolymers (>17nt), unknown bases and overrepresented sequences (more than two times the observed average frequency) were removed.
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