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Experimental results were averaged replicates of two or more data points.
All the data reported in this study are averaged replicates of two data points.
The appropriate colour of a tissue is determined by evaluating the ratio of the averaged replicates to the positive or negative maximum and converting it to the equivalent place on the colour scale: yellow to red for positive numbers and yellow to blue for negative.
PCA of averaged replicates.
SVM Classification of averaged replicates.
A comparison of a single COHCOA-analyzed MOHCA-seq data set to a single MOHCA-gel experiment is shown in Figure 2F G, and a final MOHCA-seq data set consisting of four averaged replicates is shown in Figure 1D.
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Vlahou et al. and Svetnik et al. used decision trees and RF, respectively, on averaged replicate data [11], [24].
Genomic relationships were assessed by hierarchical clustering using the Pearson correlation clustering parameter with average linkage on averaged replicate samples.
For each transcript, we then averaged replicate observations (mean) to reduce the influence of experimental variation upon the clustering analysis.
Reported values and errors were derived from software-assisted regression analyses of averaged replicate (at least triplicate) data sets.
Differentially expressed genes were identified by the Wilcoxon rank sum test after averaging replicates.
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